基于微生物矿化的生态修复措施下边坡土壤理化特性与微生物群落的协同演变研究

The synergistic evolution of soil physicochemical properties and microbial communities in slope soils with the ecological restoration measures based on Microbially Induced Carbonate Precipitation

  • 摘要: 本研究聚焦基于微生物矿化的生态修复措施(微生物诱导碳酸盐沉积-厚层基材喷射)对边坡土壤的影响, 重点解析了土壤理化性质与微生物群落的协同演变机制。研究发现, 该生态修复措施(XF)显著改善了土壤理化特性, 与对照组相比, 土壤pH由4.30升至5.20, 有机质含量由8.06 g·kg−1增至15.67 g·kg−1, 全氮含量由0.32 g·kg−1增至0.53 g·kg−1, 碱解氮含量由24.37 mg·kg−1增至43.60 mg·kg−1, 有效磷含量由0.27 mg·kg−1增至15.88 mg·kg−1, 速效钾含量由70.00 mg·kg−1增至110.67 mg·kg−1, 土壤肥力状况大幅提高。α多样性指数显示, XF组微生物群落物种丰富度更高, 且β多样性分析进一步证实XF组与对照组微生物群落组成存在显著差异。在微生物群落组成方面, XF组中放线菌门(Actinobacteriota)、γ-变形菌纲(γ-Proteobacteria)、蓝细菌门(Cyanobacteria)和拟杆菌门(Bacteroidota)等占比升高。其中, 慢生根瘤菌属(Bradyrhizobium)、伯克霍尔德氏菌-卡巴莱罗尼亚菌-副伯克霍尔德氏菌(Burkholderia-Caballeronia-Paraburkholderia)菌群和中华单胞菌属(Sinomonas)等有益微生物的相对丰度显著增加, 进而增强了有机物分解和碳氮养分循环功能。相比之下, 对照组以绿弯菌门(Chloroflexi)和酸杆菌门(Acidobacteriota)等维持土壤基础功能的土著微生物为主。利用基于距离的冗余分析, 确定有效磷为最关键环境因子, 其解释度高达98.34%。物种与环境因子相关性分析显示, 未分类的B12-WMSP1目下的属与碱解氮含量呈极显著负相关, 分枝杆菌属(Mycobacterium)与碱解氮和有机质含量均呈极显著正相关, JG30-KF-CM45科下未分类属与有效磷含量呈显著正相关。这些发现阐明了该生态修复措施下边坡土壤理化特性与微生物群落的协同演变规律, 为边坡生态修复工程提供了重要的理论依据与实践指导, 对推动生态修复技术的优化和应用具有重要意义。

     

    Abstract: This study focused on the impacts of ecological restoration measures based on MICP-TBS (Microbial Induced Carbonate Precipitation-Thick-layer Basematerial Spraying) on slope soils, with an emphasis on elucidating the synergistic evolutionary mechanisms between soil physicochemical properties and microbial communities. The findings revealed that ecological restoration measures (XF), improved the soil physicochemical characteristics. Compared to the control group, soil pH increased from 4.30 to 5.20, organic matter content increased from 8.06 to 15.67 g·kg−1, total nitrogen content increased from 0.32 to 0.53 g·kg−1, alkali-hydrolyzable nitrogen content increased from 24.37 to 43.60 mg·kg−1, available phosphorus content increased from 0.27 to 15.88 mg·kg−1, and available potassium content increased from 70.00 to 110.67 mg·kg−1, indicating a significant enhancement in soil fertility. α diversity indices demonstrated that XF treatment resulted in higher species richness in the microbial community. β diversity analysis further confirmed significant differences in microbial community composition between the XF and control treatments. The XF treatment showed enrichment of Actinobacteriota, γ-Proteobacteria, Cyanobacteria, and Bacteroidota. Notably, the relative abundances of beneficial microorganisms, such as Bradyrhizobium, Burkholderia-Caballeronia-Paraburkholderia, and Sinomonas significantly increased, thereby enhancing organic matter decomposition and carbon-nitrogen nutrient cycling. In contrast, the control group was dominated by indigenous microorganisms such as Chloroflexi and Acidobacteriota, which maintain basic soil functions. Available phosphorus content was determined to be the most crucial environmental factor through distance-based redundancy analysis, with explanatory power as high as 98.34%. Correlation analysis between species and environmental factors revealed that unclassified genera in the B12-WMSP1 order were extremely significantly negatively correlated with alkali-hydrolyzable nitrogen, whereas Mycobacterium showed extremely significant positive correlations with alkali-hydrolyzable nitrogen and organic matter content. Unclassified genera under the JG30-KF-CM45 family were significantly positively correlated with available phosphorus. These findings clarify the synergistic evolutionary patterns of soil physicochemical properties and microbial communities under ecological restoration measures, while also provide important theoretical foundations and practical guidance for slope ecological restoration projects. This study has significant implications for advancing the optimization and application of ecological restoration technologies.

     

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