Characteristics of root-associated microbiomes and their responses to soil nitrogen levels in different wheat cultivars

ZHENG Yuchong; ZHANG Linqi; LIU Binbin

doi:10.12357/cjea.20230069

Volume 31 Issue 11

Nov. 2023

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Chinese Journal of Eco-Agriculture > 2023 > 31(11): 1708-1720. > DOI: 10.12357/cjea.20230069 CSTR: 32371.14.cjea.20230069

ZHENG Y C, ZHANG L Q, LIU B B. Characteristics of root-associated microbiomes and their responses to soil nitrogen levels in different wheat cultivars[J]. Chinese Journal of Eco-Agriculture, 2023, 31(11): 1708−1720. DOI: 10.12357/cjea.20230069

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Characteristics of root-associated microbiomes and their responses to soil nitrogen levels in different wheat cultivars

1.
Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences / Hebei Key Laboratory of Soil Ecology / Key Laboratory of Agricultural Water Resources, Chinese Academy of Sciences, Shijiazhuang 050022, China
2.
University of Chinese Academy of Sciences, Beijing 100049, China

Funds: The study was supported by the National Natural Science Foundation of China (U22A60009) and the National Key Research and Development Program of China (2021YFF1000403).

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Corresponding author:
LIU Binbin, E-mail: binbinliu@sjziam.ac.cn
Received Date: February 10, 2023
Accepted Date: May 15, 2023
Available Online: August 09, 2023

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Abstract

Abstract

Plant root-associated microorganisms play important roles in nutrient uptake and plant growth. In order to illustrate the differences in the root-associated microbial community structure of different wheat cultivars, four wheat cultivars (i.e., ‘Kenong 9204’ ‘Kenong 2011’ ‘Jing 411’ and ‘Bainong 207’) were planted under 0 kg(N)·hm⁻² (low nitrogen level) and 300 kg(N)·hm⁻² (high nitrogen level), and the rhizosphere and root samples were collected at the tillering, jointing, and filling stages. The bacterial diversity and community structure in the rhizosphere and root endosphere of different wheat cultivars were analyzed using 16S rRNA high-throughput sequencing, and the physiological parameters of wheat were determined. Compared with the other three cultivars, ‘Ke-nong 9204’ had higher aboveground nitrogen accumulation at the three growth stages and under two nitrogen levels, except at the tillering stage with a low nitrogen level. Proteobacteria and Actinobacteria were the dominant bacteria in the wheat rhizosphere and root endosphere. Compared to the other three cultivars, ‘Kenong 9204’ enriched Rhizobiales and Gemmatimonas in the rhizosphere soil bacterial community under low nitrogen level at the jointing stage and enriched Frankiales under high nitrogen level at the filling stage. Correlation analysis showed that Arthrobacter, Streptomyces, Rubrobacter, and Nocardioides in the rhizosphere soil bacterial communities were significantly positively correlated with aboveground biomass and nitrogen accumulation; Massilia, Arenimonas, Pseudomonas, and Flavobacterium were significantly positively correlated with aboveground nitrogen content. Our results indicate that wheat may affect nutrient uptake by regulating the composition of the microbial community in the root zone and that this effect is cultivar-specific. This study provides useful information for understanding plant-microbe interactions in wheat and harnessing beneficial microbes for agricultural production.
- Wheat,
- Root zone microbial community,
- High-throughput sequencing,
- Aboveground nitrogen accumulation

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Characteristics of root-associated microbiomes and their responses to soil nitrogen levels in different wheat cultivars

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