Abstract: The mycelial respiration of several kinds of edible fungi was studied by employing close-road experimental equipment It was proved in our experiment that at low concentration，the CO₂ emission rate of all edible fungi were relarive higher，the order of CO₂ emission rate from high to low was Volvariella volvacea，Flammulina velutipes，Pleurotus ostreatus，Hericium erinaceus，Ganoderma lucidum and Grifola frondosa．The CO₂ ernission rate decreased at high concentration because the mycelial respiration rate was distinctly inhibited．With the same period the CO₂，emission rate of Grifola frondosa was the lowest among all edible fungi studied．