王倡宪, 李晓林, 宋福强, 王贵强, 李北齐. 两种丛枝菌根真菌对黄瓜苗期枯萎病的防效及根系抗病相关酶活性的影响[J]. 中国生态农业学报(中英文), 2012, 20(1): 53-57. DOI: 10.3724/SP.J.1011.2012.00053
引用本文: 王倡宪, 李晓林, 宋福强, 王贵强, 李北齐. 两种丛枝菌根真菌对黄瓜苗期枯萎病的防效及根系抗病相关酶活性的影响[J]. 中国生态农业学报(中英文), 2012, 20(1): 53-57. DOI: 10.3724/SP.J.1011.2012.00053
WANG Chang-Xian, LI Xiao-Lin, SONG Fu-Qiang, WANG Gui-Qiang, LI Bei-Qi. Effects of arbuscular mycorrhizal fungi on fusarium wilt and disease resistance-related enzyme activity in cucumber seedling root[J]. Chinese Journal of Eco-Agriculture, 2012, 20(1): 53-57. DOI: 10.3724/SP.J.1011.2012.00053
Citation: WANG Chang-Xian, LI Xiao-Lin, SONG Fu-Qiang, WANG Gui-Qiang, LI Bei-Qi. Effects of arbuscular mycorrhizal fungi on fusarium wilt and disease resistance-related enzyme activity in cucumber seedling root[J]. Chinese Journal of Eco-Agriculture, 2012, 20(1): 53-57. DOI: 10.3724/SP.J.1011.2012.00053

两种丛枝菌根真菌对黄瓜苗期枯萎病的防效及根系抗病相关酶活性的影响

Effects of arbuscular mycorrhizal fungi on fusarium wilt and disease resistance-related enzyme activity in cucumber seedling root

  • 摘要: 由尖孢镰刀菌引起的黄瓜枯萎病是设施黄瓜生产的主要障碍之一, 丛枝菌根真菌(AM真菌)可以和包括黄瓜在内约80%的维管植物的根系形成菌根共生体, 适宜的共生体组合对于寄主的生长与抗病性的提高十分有益。为明确Glomus versiformeGlomus intraradices两种AM真菌对“津绿3号”黄瓜苗期枯萎病的防治效果。试验采用盆钵培养的方法, 研究了两种AM真菌对幼苗生长及其根系3种抗病相关酶活性的影响。结果表明: 两种AM真菌均可促进黄瓜幼苗的生长, 并能减轻病害, 但以G. versiforme的促生及生防作用更显著, 接种G. versiforme处理的黄瓜幼苗株高、茎粗、叶面积及干重均显著大于对照, 该处理的幼苗病情指数较对照降低26.6%。菌根化黄瓜幼苗抗病性的提高一方面与接种病原菌Fusarium oxysporum f. sp. cucumerinum前幼苗生长健壮有关; 另一方面与根系抗病相关酶活性的提前诱导有关。接种F. oxysporum f. sp. cucumerinum前, G. versiforme处理的黄瓜幼苗壮苗指数显著高于G. intraradices处理与对照, 分别为G. intraradices处理与对照的1.19倍与1.22倍; G. versiforme处理的黄瓜幼苗根系几丁质酶、β-1,3-葡聚糖酶与PAL酶分别比对照提前2 d、7 d、7 d被诱导, 且酶活性分别为对照的1.44倍、2.16倍和92.00倍。

     

    Abstract: Fusarium.oxysporum f. sp. cucumerinum wilt disease severely limits cucumber production. There is a symbiotic relationship between arbuscular mycorrhizal fungus and over 80% of vascular plants including cucumber. This relationship is not only beneficial to growth, but also to tolerance of plant to pathogens. A pot experiment was conducted to investigate the bio-control effects of Glomus versiforme and Glomus intraradices against cucumber wilt. Meanwhile, the activities of three disease resistance enzymes in the root were examined. The results showed that the arbuscular mycorrhizal fungi improved cucumber seedling growth and reduced disease severity. G. versiforme presented more obviously growth-promoting and bio-controlling effects than G. intraradices. Compared with the control, G. versiforme significantly enhanced cucumber growth parameters such as seedling height, stem diameter, leaf area and dry weight while reducing disease index by 26.6%. Improved growth and early induction of defense enzymes of mycorrhizal seedlings increased plant disease resistance. Before F. oxysporum f. sp. cucumerinum infection, the main growth parameters (e.g., height, leaf area and dry weight) of G. versiforme or G. intraradices inoculated seedlings were significantly higher than those of the control. The index of seedling quality of G. versiforme inoculated seedlings was respectively 1.19 and 1.22 times of that of G. intraradices inoculated seedlings and the control seedlings. This implied that before F. oxysporum f. sp. Cucumerinum infection, G. versiforme inoculated seedlings were significantly stronger than both the G. intraradices inoculated seedlings and the control seedlings. The highest activities of chitinase, β-1,3-glucanase and PAL in G. versiforme pre-inoculated roots occurred 2, 7 and 7 days earlier than those in the control. The corresponding enzymes activities were 1.44, 2.16 and 92.00 times that of the control, respectively. The results provided efficient ways of protecting cucumber seedlings against Fusarium wilt by inducing arbuscular mycorrhizal fungi in cucumber seedlings.

     

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