郑小亚, 张正斌, 徐萍, 杨引福. 逆境条件下糯玉米水分利用效率和水通道蛋白表达差异研究[J]. 中国生态农业学报(中英文), 2011, 19(3): 607-613. DOI: 10.3724/SP.J.1011.2011.00607
引用本文: 郑小亚, 张正斌, 徐萍, 杨引福. 逆境条件下糯玉米水分利用效率和水通道蛋白表达差异研究[J]. 中国生态农业学报(中英文), 2011, 19(3): 607-613. DOI: 10.3724/SP.J.1011.2011.00607
ZHENG Xiao-Ya, ZHANG Zheng-Bin, XU Ping, YANG Yin-Fu. Differences in water use efficiency and aquaporin gene expression in waxy maize varieties under different stress conditions[J]. Chinese Journal of Eco-Agriculture, 2011, 19(3): 607-613. DOI: 10.3724/SP.J.1011.2011.00607
Citation: ZHENG Xiao-Ya, ZHANG Zheng-Bin, XU Ping, YANG Yin-Fu. Differences in water use efficiency and aquaporin gene expression in waxy maize varieties under different stress conditions[J]. Chinese Journal of Eco-Agriculture, 2011, 19(3): 607-613. DOI: 10.3724/SP.J.1011.2011.00607

逆境条件下糯玉米水分利用效率和水通道蛋白表达差异研究

Differences in water use efficiency and aquaporin gene expression in waxy maize varieties under different stress conditions

  • 摘要: 水肥耦合是作物水分和养分高效利用的重要机制, 其中水通道蛋白(AQP)发挥重要作用。本文研究了逆境条件下3 个糯玉米品种水分利用效率(WUE)和水通道蛋白(AQP)表达差异及其关系。在低氮(0.5N, 其中培养液的氮元素含量减半)、高氮(1.5N, 其中培养液的氮元素含量增加1/2)、干旱(10% PEG 即-0.1 MPa)和盐(200 mmol·L-1 NaCl)胁迫处理下测定了叶片水分利用效率(WUEL), 并采用半定量逆转录聚合酶链式反应(RT-PCR)体系检测3 个AQP 基因在糯玉米根系中的表达情况。结果表明: (1) 0.5N 处理下, 3 个品种的WUEL 多低于CK;1.5N 和PEG 处理下, 3 个品种的WUEL 多高于CK。(2)TIP3.1、TIP2a、TIP2b 3 个基因在0.5N 处理下表现为不表达或表达下调, 1.5N 处理下6 h 和PEG 处理下9 h 多表现表达上调, 说明TIPs 基因家族表达受营养、干旱和盐胁迫诱导。(3)TIPs 的上述3 个基因的表达和WUEL 都在0.5N 处理下低于CK, 在1.5N 和PEG 处理下不同程度地高于CK, 说明TIPs 基因表达与WUEL 的变化有密切响应关系。以上结果说明WUE 与TIPs 基因转录水平的调控有一定关系, 且品种间存在较大差异。

     

    Abstract: Aquaporin (AQP) is critical in coupling water and nutrients, which is an important mechanism of crop water (WUE) and nutrient (NUE) use efficiency. Differences in leaf water use efficiency (LWUE) and in aquaporin gene expression in waxy maize varieties were tested under various stress conditions. The stress conditions used in the study included low nitrogen (0.5N, half dose nitrogen of 0.5 Hoagland solution), high nitrogen (1.5N, 1.5 times nitrogen of 0.5 Hoagland solution), drought (10% PEG with -0.1 MPa water potential ) and salt (200 mmol·L-1 NaCl). Expressions of three AQP genes in the roots of waxy maize were tested by the RT-PCR method. The results showed that LWUE in three waxy maize varieties was less under 0.5N treatment than that under CK. It was, however, higher than that of CK under 1.5N and PEG treatments. TIP2a, TIP2b and TIP3.1 genes expressions were partially or completely suppressed under 0.5N stress treatment. The gene expressions were, however, amplified under 1.5N (for 6 h) and PEG (for 9 h) treatments. This suggested that TIP gene family was enhanced by nitrogen, drought and salt stress. Furthermore, the expression of TIP and LWUE were weaker under 0.5N treatment than under CK, but stronger on different degrees under PEG and 1.5N treatments than under CK. Close relations were noted between TIP gene expression and change in WUEL. These findings indicated that WUE was largely controlled by the levels of TIP expression, which was different among waxy maize varieties.

     

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