古少鹏, 郑明学, 李宝钧, 张春善, 申红星. 铜和维生素A 及其互作效应对肉鸡免疫功能的影响[J]. 中国生态农业学报(中英文), 2011, 19(1): 135-140. DOI: 10.3724/SP.J.1011.2011.00135
引用本文: 古少鹏, 郑明学, 李宝钧, 张春善, 申红星. 铜和维生素A 及其互作效应对肉鸡免疫功能的影响[J]. 中国生态农业学报(中英文), 2011, 19(1): 135-140. DOI: 10.3724/SP.J.1011.2011.00135
GU Shao-Peng, ZHENG Ming-Xue, LI Bao-Jun, ZHANG Chun-Shan, SHEN Hong-Xing. Influence of dietary copper and vitamin A on immune function of broilers[J]. Chinese Journal of Eco-Agriculture, 2011, 19(1): 135-140. DOI: 10.3724/SP.J.1011.2011.00135
Citation: GU Shao-Peng, ZHENG Ming-Xue, LI Bao-Jun, ZHANG Chun-Shan, SHEN Hong-Xing. Influence of dietary copper and vitamin A on immune function of broilers[J]. Chinese Journal of Eco-Agriculture, 2011, 19(1): 135-140. DOI: 10.3724/SP.J.1011.2011.00135

铜和维生素A 及其互作效应对肉鸡免疫功能的影响

Influence of dietary copper and vitamin A on immune function of broilers

  • 摘要: 采用4×2(铜×维生素A)完全随机试验设计, 研究了日粮添加不同水平的铜(0 mg·kg-1、8 mg·kg-1、150 mg·kg-1、225 mg·kg-1)和维生素A(1 500 IU·kg-1、5 000 IU·kg-1)对肉仔鸡不同生长阶段(0~4 周龄和5~7 周龄)免疫功能的影响。结果表明: 添加铜为8 mg·kg-1 时, 肉鸡生长前期(0~4 周龄)可显著(P<0.05)或极显著(P<0.01)提高肉鸡脾脏活化T 淋巴细胞百分率和血清抗体效价, 生长后期(5~7 周龄)可极显著(P<0.01)或显著(P<0.05)提高脾脏、胸腺、盲肠扁桃体活化T 淋巴细胞百分率和外周血液中活化T、B 淋巴细胞百分率; 整个试验期添加高铜(150 mg·kg-1、225 mg·kg-1)时, 活化T、B 淋巴细胞百分率均不同程度下降。添加维生素A 5 000 IU·kg-1 时, 肉鸡生长前期可显著(P<0.05)提高其外周血液中活化T 淋巴细胞百分率, 生长后期可极显著(P<0.01)或显著(P<0.05)提高脾脏、盲肠扁桃体活化T 淋巴细胞百分率和血清抗体效价。铜和维生素A 互作效应对肉鸡生长前期的活化T、B 淋巴细胞百分率和血清抗体效价影响不显著, 对生长后期的外周血液、胸腺、盲肠扁桃体活化T 淋巴细胞百分率影响显著(P<0.05), 以8 mg·kg-1 铜× 5 000 IU·kg-1 维生素A 处理最高; 以上结果提示铜和维生素A 对肉鸡免疫功能的影响存在互作效应。

     

    Abstract: A 4×2 (Cu×VA) random experiment was designed to investigate the effect of different dietary levels of Cu (0 mg·kg-1, 8 mg·kg-1, 150 mg·kg-1, 225 mg·kg-1) and VA (1 500 IU·kg-1, 5 000 IU·kg-1) on broiler immune system at 0~4 and 5~7 week-old. The results showed significant effects (P<0.05, P<0.01) of Cu at 8 mg·kg-1 on percentage of activated T-lymphocytes in spleen and serum antibody titers of 0~4 week-old broilers. At the same Cu level, percentage of activated T-lymphocytes in spleen, thymus and cecal tonsil, and percentage of activated B/T-lymphocytes in peripheral blood significantly increased (P<0.05, P<0.01) in 5~7 week-old broilers. Percentage of activated B/T-lymphocytes decreased at different degrees under high Cu levels of 150~225 mg·kg-1 feed. At VA supplemental level of 5 000 IU·kg-1 feed, percentage of activated T-lymphocytes in peripheral blood also significantly increased (P<0.05) for the 0~4 week-old broilers, while percentage of activated T-lymphocytes in spleen, cecal tonsil and serum antibody titers significantly increased (P<0.05, P<0.01) for the 5~7 week-ld broilers. Percentage of activated B/T-lymphocytes and serum antibody titers were not significantly affected by Cu/VA interaction for 0~4 week-old broilers. However, percentage of activated T-lymphocytes of cecal tonsil, thymus and peripheral blood significantly increased (P<0.05) for 5~7 week-old broilers, especially under Cu (8 mg·kg-1) × VA (5 000 IU·kg-1) interaction. Cu/VA interaction had a significant effect on the function of broiler immune system.

     

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