邱秋金, 李圆萍, 王微, 陈珊, 肖清铁, 郑新宇, 王经源, 林瑞余, 林文雄. 看麦娘叶片对化感小麦根水提液的生理响应[J]. 中国生态农业学报(中英文), 2014, 22(11): 1357-1363. DOI: 10.13930/j.cnki.cjea.140470
引用本文: 邱秋金, 李圆萍, 王微, 陈珊, 肖清铁, 郑新宇, 王经源, 林瑞余, 林文雄. 看麦娘叶片对化感小麦根水提液的生理响应[J]. 中国生态农业学报(中英文), 2014, 22(11): 1357-1363. DOI: 10.13930/j.cnki.cjea.140470
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QIU Qiujin, LI Yuanping, WANG Wei, CHEN Shan, XIAO Qingtie, ZHENG Xinyu, WANG Jingyuan, LIN Ruiyu, LIN Wenxiong. Physiological response of Alopecurus aequalis leaf to water extracts from allelopathic wheat (Triticum aestivum L.) root tissues[J]. Chinese Journal of Eco-Agriculture, 2014, 22(11): 1357-1363. DOI: 10.13930/j.cnki.cjea.140470
Citation: QIU Qiujin, LI Yuanping, WANG Wei, CHEN Shan, XIAO Qingtie, ZHENG Xinyu, WANG Jingyuan, LIN Ruiyu, LIN Wenxiong. Physiological response of Alopecurus aequalis leaf to water extracts from allelopathic wheat (Triticum aestivum L.) root tissues[J]. Chinese Journal of Eco-Agriculture, 2014, 22(11): 1357-1363. DOI: 10.13930/j.cnki.cjea.140470
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看麦娘叶片对化感小麦根水提液的生理响应

Physiological response of Alopecurus aequalis leaf to water extracts from allelopathic wheat (Triticum aestivum L.) root tissues

    摘要
  • 摘要: 为阐明小麦化感抑草的生理机制, 选择强化感小麦'115/青海麦'、'92L89'和弱化感小麦'抗10103', 通过添加浓度为0.2%、0.5%和1.0%的小麦根水提液进行水培试验3周后, 测定了各处理看麦娘的鲜重, 分析叶片中叶绿素(SPAD值)、可溶蛋白、MDA、类黄酮、总酚的含量以及SOD、POD、CAT活性。结果表明, 水提液处理显著抑制了看麦娘的生长, 抑制率在不同处理浓度及小麦品种间均存在显著差异, 强化感小麦的抑制率显著高于弱化感小麦。在处理浓度范围内, 不同小麦根水提液的抑制率大小依次为'115/青海麦'(24.7%~ 74.3%)>'92L89'(15.7%~71.6%)>'抗10103'(13.8%~61.4%); 0.2%、1.0%和5.0%水提液处理的抑制率大小依次为13.8%~24.7%、41.7%~66.4%和61.4%~74.2%。看麦娘叶绿素含量(SPAD值)随处理浓度增大显著降低, 可溶蛋白含量, SOD、POD、CAT活性, MDA、类黄酮含量随处理浓度增大显著升高, 强化感小麦对看麦娘的生理刺激作用高于弱化感小麦。1.0%'115/青海麦'及5.0%各小麦水提液处理的看麦娘总酚含量高于对照。可见, 小麦化感胁迫提高了看麦娘的保护酶系统活性, 增强了抗氧化物质代谢, 但显著增强了细胞膜脂质过氧化和叶绿素降解, 不利于靶标植物看麦娘的生长。

     

    Abstract: In order to illustrate the physiological mechanism of weed suppression ability of allelopathic wheat (Triticum aestivum L.), two strong allelopathic wheat accessions ('115/Qinghai' and '92L89') and one weak allelopathic accession ('Kang10103') were used in hydroponic culture experiments. Alopecurus aequalis seedlings were cultured in the hydroponic culture solutions with 0.2%, 0.5% and 1.0% of roots water extracts of the above three wheat accessions. The fresh weights, contents of chlorophyll (SPAD value), soluble proteins, methane dicarboxylic aldehyde (MDA), flavonoids, total phenols and the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) of A. aequalis seedlings were determined after cultured for 21 days. The results showed significant differences in weed suppression ability among wheat accessions and concentrations of roots water extracts. Strong allelopathic wheat accessions had higher inhibitory rates to A. aequalis seedlings than weak allelopathic wheat accessions. The inhibitory rate to A. aequalis seedling fresh weight of different wheat root water extracts within designed concentrations was in the order of '115/Qinghai' (24.7%-74.3%) > '92L89' (15.7%-71.6%) > 'Kang10103' (13.8%-61.4%). Wheat accessions with extract concentrations of 0.2%, 1.0% and 5.0% had inhibitory rates of 13.8%-24.7%, 41.7%-66.4% and 61.4%-74.2%, respectively. The contents of chlorophyll decreased with increasing extract concentration while the contents of soluble protein, MDA, flavonoids, and activities of SOD, POD and CAT increased with decreasing extract concentration. Strong allelopathic wheat accessions had higher stimulation effects on physiological indexes than weak wheat accessions. In addition, total phenol content of A. aequalis seedling treated with 1.0% water extract of '115/Qinghai' wheat and 5.0% water extracts of the three wheat accessions were higher than the control. Thus allelopathy effects of wheat root water extracts enhanced enzyme activities, stimulated metabolism of anti-oxidatives, accompanied stronger lipid peroxidation, degraded leaf chlorophyll and inhibited growth of A. aequalis seedling.

     

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