丁凯鑫, 王立春, 田国奎, 王海艳, 李凤云, 潘阳, 庞泽, 单莹. 干旱胁迫下不同品种马铃薯块茎膨大期叶片对烯效唑的生理响应[J]. 中国生态农业学报 (中英文), 2023, 31(7): 1067−1080. DOI: 10.12357/cjea.20230184
引用本文: 丁凯鑫, 王立春, 田国奎, 王海艳, 李凤云, 潘阳, 庞泽, 单莹. 干旱胁迫下不同品种马铃薯块茎膨大期叶片对烯效唑的生理响应[J]. 中国生态农业学报 (中英文), 2023, 31(7): 1067−1080. DOI: 10.12357/cjea.20230184
DING K X, WANG L C, TIAN G K, WANG H Y, LI F Y, PAN Y, PANG Z, SHAN Y. Physiological responses of leaves of different potato varieties to uniconazole during tuber expansion stage under drought stress[J]. Chinese Journal of Eco-Agriculture, 2023, 31(7): 1067−1080. DOI: 10.12357/cjea.20230184
Citation: DING K X, WANG L C, TIAN G K, WANG H Y, LI F Y, PAN Y, PANG Z, SHAN Y. Physiological responses of leaves of different potato varieties to uniconazole during tuber expansion stage under drought stress[J]. Chinese Journal of Eco-Agriculture, 2023, 31(7): 1067−1080. DOI: 10.12357/cjea.20230184

干旱胁迫下不同品种马铃薯块茎膨大期叶片对烯效唑的生理响应

Physiological responses of leaves of different potato varieties to uniconazole during tuber expansion stage under drought stress

  • 摘要: 为明确外源烯效唑对干旱胁迫下马铃薯的缓解效应, 以耐旱品种‘克新1号’和干旱敏感品种‘大西洋’为试验材料, 在块茎膨大期(第二花序开花)进行干旱处理(保持45.0%的土壤相对含水量)和喷施烯效唑(40 mg∙L−1), 每个品种各设3个处理: 对照(CK)、干旱胁迫(D)和干旱胁迫+烯效唑(D+S), 测定外源烯效唑和干旱胁迫下马铃薯叶片叶绿素含量、光合气体交换参数、细胞膜脂过氧化程度(MDA)、活性氧和抗氧化防御系统中抗氧化酶活性以及抗坏血酸-谷胱甘肽循环产物和底物的含量。结果表明, 干旱胁迫能够降低2个品种叶片的叶绿素含量, 显著抑制净光合速率、蒸腾速率、气孔导度、胞间CO2浓度, 而显著增加MDA、H2O2含量和O2产生速率。在干旱胁迫15 d时, D处理的‘克新1号’和‘大西洋’叶片的MDA、H2O2含量、O2产生速率分别较CK显著增加29.2%和43.6%、55.6%和73.1%、84.3%和110.6%。2个品种抗氧化酶活性和非酶抗氧化剂含量均有所升高, 且耐旱品种‘克新1号’受干旱损伤程度小于干旱敏感品种‘大西洋’。外源烯效唑能够显著增加干旱胁迫下马铃薯叶片的叶绿素含量和光合气体交换参数, 降低‘克新1号’和‘大西洋’叶片内MDA、O2产生速率和H2O2含量, 同时提高抗氧化防御系统中抗氧化酶活性; 烯效唑可进一步上调2个马铃薯品种干旱胁迫下抗坏血酸(AsA)、脱氢抗坏血酸(DHA)、谷胱甘肽(GSH)和氧化型谷胱甘肽(GSSG)含量, 并提高AsA/DHA和GSH/GSSG比值 。综上, 外源烯效唑可在一定程度上促进叶片光合作用, 提高抗氧化防御能力, 缓解干旱胁迫所造成的损伤。研究结果可为抗旱栽培提供理论依据。

     

    Abstract: To clarify the alleviating effect of exogenous uniconazole on potatoes under drought stress, the drought-tolerant variety ‘KX1’ and the drought-sensitive variety ‘Atlantic’ were used as experimental materials. Drought treatment (maintaining 45% relative soil water content) and spraying uniconazole (40 mg·L−1) were carried out at the tuber expansion stage (second inflorescence flowering). Three treatments were established for each variety: control (CK), drought stress (D), and drought stress + uniconazole (D+S). The chlorophyll content, photosynthetic parameters, membrane lipid peroxidation (malonaldehyde, MDA), reactive oxygen species (ROS), and antioxidant enzyme activity in the antioxidant defense system, as well as ascorbate aicd–glutathione (AsA-GSH) cycle products and substrate content in potato leaves under exogenous uniconazole and drought stress were measured. The results showed that drought stress reduced the chlorophyll content of the two varieties leaves, and significantly inhibited net photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs), intercellular CO2 concentration (Ci), but significantly increased MDA, H2O2 content, and O2 production rate. On the 15th day of drought stress, MDA and H2O2 contents, and O2 production rate in D treatment of ‘KX1’ and ‘Atlantic’ leaves were significantly increased by 29.2% and 43.6%, 55.6% and 73.1%, 84.3% and 110.6%, respectively, compared to CK; the antioxidant enzyme activity and non-enzymatic antioxidant content of both varieties increased. The drought-tolerant variety ‘KX1’ was less affected by drought than the drought-sensitive variety ‘Atlantic’. Exogenous uniconazole significantly increased the chlorophyll content and photosynthetic parameters of potato leaves, reduced the MDA content, O2 production rate, and H2O2 content in leaves of ‘KX1’ and ‘Atlantic’, and increased the activity of antioxidant enzymes in the antioxidant defense system. In addition, uniconazole further increased the contents of ascorbic acid (AsA), dehydroascorbate (DHA), glutathione (GSH), and oxidized glutathione (GSSG) in the AsA-GSH cycle of the two potato varieties under drought stress and increased the ratio of AsA/DHA and GSH/GSSG. In conclusion, exogenous uniconazole can promote leaf photosynthesis to a certain extent and improve the antioxidant defense capacity to alleviate the damage caused by drought stress. These results provide a theoretical basis for drought-tolerant cultivation.

     

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