熊勇华, 许杨, 赖卫华. 红曲霉SAGE文库中双标签的制备研究[J]. 中国生态农业学报(中英文), 2004, 12(1): 19-22.
引用本文: 熊勇华, 许杨, 赖卫华. 红曲霉SAGE文库中双标签的制备研究[J]. 中国生态农业学报(中英文), 2004, 12(1): 19-22.
shu
X10NG Yong-Hua, XU Yang, LAI Wei-Hua. Preparation of ditags in constructing SAGE library of Monasccus Ruber[J]. Chinese Journal of Eco-Agriculture, 2004, 12(1): 19-22.
Citation: X10NG Yong-Hua, XU Yang, LAI Wei-Hua. Preparation of ditags in constructing SAGE library of Monasccus Ruber[J]. Chinese Journal of Eco-Agriculture, 2004, 12(1): 19-22.
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红曲霉SAGE文库中双标签的制备研究

Preparation of ditags in constructing SAGE library of Monasccus Ruber

    摘要
  • 摘要: 研究探讨了红曲霉SAGE文库构建过程中总RNA提取、mRNA分离纯化、cDNA合成以及双标签(Ditags)制备方法，并通过PCR条件的优化获得双标签PCR的最佳模板稀释浓度及扩增循环数。

     

    Abstract: In this paper，the methods of total RNA and mRNA extraction，cDNA synthesis and ditags preparation are discussed in constructing SAGE library of Monasccus Ruher. The optima1 concentration of template and the number of PCR cycles are obtained by optimizing the PCR conditions．

     

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