徐洪伟, 周晓馥. 高山红景天毛状根培养的研究[J]. 中国生态农业学报(中英文), 2003, 11(3): 45-47.
引用本文: 徐洪伟, 周晓馥. 高山红景天毛状根培养的研究[J]. 中国生态农业学报(中英文), 2003, 11(3): 45-47.
XU Hong-Wei, ZHOU Xiao-Fu. Research of the hairy-root of Rhodiola sachalinensis A.Bor.[J]. Chinese Journal of Eco-Agriculture, 2003, 11(3): 45-47.
Citation: XU Hong-Wei, ZHOU Xiao-Fu. Research of the hairy-root of Rhodiola sachalinensis A.Bor.[J]. Chinese Journal of Eco-Agriculture, 2003, 11(3): 45-47.

高山红景天毛状根培养的研究

Research of the hairy-root of Rhodiola sachalinensis A.Bor.

  • 摘要: 建立了高山红景天毛状根培养系统,研究表明红景天子叶、子叶节能被A4、R1601、ATCC15834感染产生毛状根,PCR检测在500bp处有rolC基因存在。以1/2MS0 液体培养基、散射光、温度为20℃左右、pH 值4.5~4.8间毛状根生长为佳。对C源要求不高,N 源NH4+ :N03- 为1:1时毛状根生长较好且红景天甙含量增加。

     

    Abstract: The hairy-root culture system of Rhodiola sachalinensis A .Bor was established in this paper.The results show that the hairy-root can be arisen at cotyledon and cotyledonary node infected by A4,R1601,ATCC15834 agrobacterium Ri-plasmid.There is about 500bp-rolC gene detected from the hairy-root culture by PCR amplifying. The hairy-root grows well at 1/2MSo liquid medium,scatter light,20℃ and pH 4.5~4.8.In the condition of the lower nitrogen substance.such as NH4+:N03- equal to 1:1,the hairy-root grows well and the content of salidrls-ide has been increased.

     

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