潘锦旭, 邢震, 郑维列. 西藏卓巴百合组织培养技术研究[J]. 中国生态农业学报(中英文), 2002, 10(2): 26-28.
引用本文: 潘锦旭, 邢震, 郑维列. 西藏卓巴百合组织培养技术研究[J]. 中国生态农业学报(中英文), 2002, 10(2): 26-28.
PAN Jin-Xu, XING Zhen, ZHENG Wei-Lie. Tissue culture of Lilium wardlii[J]. Chinese Journal of Eco-Agriculture, 2002, 10(2): 26-28.
Citation: PAN Jin-Xu, XING Zhen, ZHENG Wei-Lie. Tissue culture of Lilium wardlii[J]. Chinese Journal of Eco-Agriculture, 2002, 10(2): 26-28.

西藏卓巴百合组织培养技术研究

Tissue culture of Lilium wardlii

  • 摘要: 对西藏野生卓巴百合(Lilium wardlii stapf)进行组织培养结果表明,选用球茎内层、基部的鳞片作为外殖体,转绿率最高;在参试的培养基中MS+BA0.5mg/kg+NAA0.5~1.0mg/kg的培养基对诱导愈伤组织(EC)和不定芽的效果较好,MS+IBA1.0mg/kg对促进小鳞茎生根效果最佳;茎盘切块在MS+BA0.2~0.5mg/kg+NAA0.5~1 0mg/kg的培养基上能正常转绿,形成愈伤组织和不定芽;活性碳有助于小鳞茎生根;组培苗移栽到松锯末中其成活率可达到95%。

     

    Abstract: Tissue culture of Lilium wardlii shows that the most perfect method of inducing EC(callus)and uncertain budlets belongs the medium of MS and BA 0.5mg/kg and NAA0.5~1.0mg/kg:the best medium inducing roots from littie bulbs is the medium of MS and IBA1.0mg/kg;the sterm cuts can be green and induce EC and uncertain budlets in the medium of MS and BA0.2~0.5mg/kg and NAA0.5~1.0mg/kg;the active carbon can help little bulbs striking roots when the tissue culture plants are moved to raise in the pine sawdust and their survival rate is 95 percent.

     

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