营养生态条件对蜜蜂球囊菌生长及产孢的影响
Effects on the mycelia growth and sporeforming of Ascosphaera apis under ecological condition of nutrients
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摘要: 从碳源、氮源、维生素、矿质元素等方面研究了营养生态条件对蜜蜂球囊菌(Ascosphaera apis Olive & Spiltoir)生长及产孢的影响。结果表明,营养生态条件的变化对蜜蜂球囊菌的影响极大。A.apis菌丝体生长最好的碳源是果糖,产孢量最高的是葡萄糖,不利用山梨糖和甘露糖;该菌优先利用有机氮源,不利用无机氮源中NaNO2、H2NCSNH2;维生素对A.apis产孢量有极为显著的影响,复合维生素比单一维生素能更好地促进菌丝体生长,尤其对产孢更为有利;矿质元素中Mg2+、P5+、K+、Zn2+促进菌丝体生长和孢子的形成,Na+对菌丝体的生长有抑制作用。Abstract: The effects on the mycelia growth and sporeforming of A.apis under ecological condition of nutrients-carbon and nitrogen sourse, vitamin and mineral elements are analyzed. The results show that the fungus grow best in D-fructose, spore-forming best in D-glucose but no growth occurs in D-mannose and D-sorbose. Except NaNO2 and H2NCSNH2,the fungus give priority to utilize organic nitrogen sources. Vitamins have distinctive promoting effect on the sporeforming of A. apis, and the effect of mixed application of several vitamins is more efficient than any single one. Mg2+、P5+、K+ and Zn2+ can promote the sporeforming and growth of the fungus, Na+ constrains the growth of the mycelia of A. apis.
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Keywords:
- Ascosphaera apis /
- Ecological condition of nutrients /
- Mycelia growth /
- Spore-forming
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