珠美海棠组培苗繁殖技术的研究
Technology of tissue cultural propagation of Malus zumi
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摘要: 以珠美海棠为材料，研究了培养基配比、苗龄、琼脂等组培苗工厂化生产关键技术，光照条件和培养容器对组培苗生长发育的影响。结果表明，采用改良型培养基MS+6-BA0.5mg/L+IAA0.2mg/L用于分化培养、1/2MS+IAA0.5mg/L或1/2MS+IBA0.5mg/L用于生根培养，苗龄30～40d，使用接近国际标准的琼脂、光照强度1500～2000lx条件下生产的组培苗繁殖系数最大、有效苗最多、生根率高及生长速度最快，移栽成活率＞90%；苗圃移栽成活率＞85%，1年生苗株高均＞1.5m。并建立了珠美海棠工厂化生产工艺及组培苗培养标准,为其他植物组培苗繁殖提供理论依据。Abstract: Culturing the stems of Malus zumi in vitro,the key techniques including medium,auxin,age of sprouts and agar are studied.The results show that if there is the advanced medium MS+6-BA 0.5mg/L+IAA0.2mg/L for multiplicating ，1/2MS+IAA0.5mg/L or 1/2MS+IBA0.5mg/L for rooting,sprout's age being 30～40 days,agar corresponding to the international standard and the light intensity sustaining at 1500～2000lx,the coefficient of multiplication,the quantity of effictive plantlets and the rate of rootage and growth are best,and the surviving ratio of transplantation of plantlets and nursery seedling is above 90% and 85% respectively,and 1-yr-old tree height is above 1.5m.Based on the study,the industrializing technology of tube-plantlets of Malus zumi and the culture standard which improves a theoretical basis for vegetive propagation of others plant are summarized.
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Keywords:
- Malus zumi /
- Tissue-culture sprout /
- Propagative technology /
- Culture medium
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