穆素梅, 钟冠昌, 李振声, 李滨. 利用缺体回交法选育小黑麦异代换系的研究[J]. 中国生态农业学报(中英文), 1996, 4(1): 33-36.
引用本文: 穆素梅, 钟冠昌, 李振声, 李滨. 利用缺体回交法选育小黑麦异代换系的研究[J]. 中国生态农业学报(中英文), 1996, 4(1): 33-36.
Mu Sumei, Zhong Guanchang, Li ZhenSheng, Li Bin. A research on nullisomic backcross method for breeding alien substitution lines[J]. Chinese Journal of Eco-Agriculture, 1996, 4(1): 33-36.
Citation: Mu Sumei, Zhong Guanchang, Li ZhenSheng, Li Bin. A research on nullisomic backcross method for breeding alien substitution lines[J]. Chinese Journal of Eco-Agriculture, 1996, 4(1): 33-36.

利用缺体回交法选育小黑麦异代换系的研究

A research on nullisomic backcross method for breeding alien substitution lines

  • 摘要: 以4D缺体为母本,黑麦为父本进行杂交,授粉后16~18d进行幼胚培养,培养的试管苗用0.05%秋水仙素处理,加倍后的植株再用缺体回交1~2次,就可获得小黑麦异代换系。在第二次回交的F1(B1F1)代里,单体代换植株占18.5%;双单体代换占24%,这两种类型经自交后都获得了4D/4R异代换系,有的异代换系已直接用于生产。

     

    Abstract: The embryoes of F1 hybrids between 4D nullisomic wheat, as female,and rye,as male,were cuhured after fertilizing 16 to 18 days,and the F1 plantlets were doubled under the treatment of 0.05% colchieine.The doubled plantlets were then backcrossed with nullisomics oneto two times.thus wheat-rye alien substitution lines could be obtained.There were about 18.5% of monosomic substitution plants and about 24.0% of double monosomic substitution in the B1F1.Aline substitution lines 4D/4R were produced by their selfing.Now some of the aline substitution lines have been applied directly to production.

     

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