Abstract: GC-MS analysis was used to identify the volatile chemical constituents of M. glyptostroboides seeds, and their toxicity to 8 plant pathogens (Alternaria kikuchiana, A. alternate, Stemphylium botryosum, Botrytis cinerea, Fusarium graminearum, Pestalotiopsis theae, Colletotrichum sp. and A. porri) was measured by mycelium growth inhibition method. The main components of M. glyptostroboides seed volatiles are 1R-alpha-Pinene (46.355%), Limonene (13.663%), Bicyclo 2.2.1 heptan-2-ol,1,7,7 -trimethyl-acetate (1S-endo) (6.941%), Caryophyllene oxide (6.924%), beta-Pinene (4.902%), Bicyclo 3.1.1 heptane,6,6-dimethyl-2-methylene (1S) (4.807%), etc. The volatiles evidently inhibit mycelium growth of 8 plant pathogenic fungi. At 100 μL·dish^-1 dose, the inhibitory rates are higher than 60% to S. botryosum, F. graminearum and P. theae. The results indicate that volatiles of M. glyptostroboides seeds have various active components. They have copious application prospects in medicine, chemical industry and the development of pesticides of agriculture.