Abstract: A pot experiment was conducted with a randomized complete block design to investigate the effects of different levels of bacterial manure on soil biochemical reaction, enzyme activity and rape yield and quality in reclaimed core-mining soils under combination application of organic and chemical fertilizers. The results show that bacterial manure increases soil biochemical reaction thereby enhancing soil enzyme activity. Soil respiration rate, ammonification and nitrification intensity, as well as catalase (CAT) activity under bacterial manure treatment are significantly (P<0.05) higher than those under the control. When the content of bacterial manure exceeds 0.04 g·kg^-1(soil), significant differences (P<0.05) are observed between the treatment and control in the activities of urease and sucrose. Besides, bacterial manure enhances rape production and quality. There are significant (P<0.05) differences between treatment and control in average dry-weight of individual plants and in contents of nitrate and reducing sugar. When the level of bacterial manure exceeds 0.04 g·kg^-1(soil), the content of Vc significantly (P<0.05) outweighs the control. When the level of bacterial manure is less than 0.08 g·kg^-1 soil, no significant differences are noted between treatment and control in fresh-weights of individual plants. The study indicates that bacterial manure positively influences soil biological activity and rape quality in reclaimed core-mining areas.