超干处理两种落叶松种子的生理生化特征研究

Physiological and biochemical characteristics of Larix olgensis and L. gmelini seeds under ultra-dry treatment

  • 摘要: 采用硅胶干燥法, 将两种落叶松种子含水量由自然含水量降至5%以下, 测定不同含水量种子老化前后的发芽势、发芽率、发芽指数、活力指数、相对电导率、脱氢酶和异柠檬酸裂解酶活性。结果表明: 长白落叶松种子含水量降至3.02%时, 种子各发芽指标在老化前后保持在较高水平, 且老化前的活力指数及老化后的发芽率、发芽指数、活力指数显著高于自然干燥种子; 在此含水量下, 老化后种子脱氢酶和异柠檬酸裂解酶活性显著高于自然干燥种子, 相对电导率比后者低, 说明长白落叶松种子以含水量为3.02%时储藏较为适宜。兴安落叶松种子含水量为2.63%时, 各发芽指标均高于自然干燥种子, 且老化前除发芽势外各发芽指标及老化后各发芽指标差异显著; 此含水量下, 老化后种子脱氢酶和异柠檬酸裂解酶活性显著高于自然干燥种子, 相对电导率比后者低, 比其他含水量种子表现出更强的抗老化能力, 说明兴安落叶松种子较适宜在2.63%含水量下储藏。

     

    Abstract: Seeds were dried to 5% moisture content (MC) or less by the silica gel method. After ultra-drying (before and after aging), germination energy (GE), germination percent (GP), germination index (GI), vigor index (VI), relative conductivity (RC), dehydrogenase (DH) and isocitratelyase (ICL) activity were investigated. The results show that GE, GP, GI, VI of ultra-dried L. olgensis seeds with 3.02% MC maintain a relatively high level before and after aging. Under 3.02% MC, VI before aging, GP, GI, VI, DH and ICL activities after aging are significantly higher, and RC is lower than those of seeds without ultra-drying treatment (natural-dry). This implies that 3.02% MC is a feasible seed moisture content during storage for L. olgensis. Ultra-dried L. gmelini seeds with 2.63% MC before and after aging keep higher germination vigor, with significant higher GP, GI, VI before aging, and GE, GP, GI, VI after aging than those of naturally dried seeds. Their DH and ICL activities are significantly higher, and RC is lower after aging compared with naturally dried seeds. L. gmelini seeds with 2.63% MC show stronger anti-aging ability than those with others levels of MC, therefore, 2.63% is feasible seed moisture content during storage for L. gmelini.

     

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