Abstract: The effect of four pesticides (acetamiprid, imidacloprid, omethoate and beta-cypermethrin) on 1000-kernel weight, yield and quality of three high quality wheat varieties (“Linyou 145”, “Linyou 2069” and “Linyou 2018”) were studied in a field experiment. Results show that pesticides increase 1000-kernel weight and yield of high quality wheat. Omethoate has higher effect than the other pesticides followed by beta-cypermethrin. However, the differences of 1000-kernel weight and yield of high quality wheat under omethoate and beta-cypermethrin treatments are insignificant. Omethoate and beta-cypermethrin increase grain protein content in three high quality wheat varieties. Almost all processing quality indices of strong gluten wheat varieties (“Linyou 145” and “Linyou 2069”) are decreased, with no notable difference from the control. Nutritional and processing quality of moderate gluten wheat (“Linyou 2018”) improves. Acetamiprid has the worst effect on yield and quality of high quality wheat. Pesticides have higher effect on stored protein, gliadin and glutelin in high quality wheat than on soluble protein, albumin and globulin.