Abstract: The limiting factors of soil nutrient in three different banana orchard farms, Dongchong, Lingshan and Wanqingsha, in Panyu District, Guangzhou City, were identified by systematic application of indicator plant (banana) and diagnostic soil analysis (soil adsorption trial and pot experiment). Results show lack of soil N in all three banana orchards in the study area. Compared with OPT (Optimal Treatment), yield of N treatment markedly decreases by 59.7%, 46.7% and 55.1% in Dongchong, Lingshan and Wanqingsha respectively. Banana K and Mg content is low, while B is high in the Dongchong and Lingshan banana orchards. P and K are lacking in soils in Wanqingsha banana orchard. However, soil Mn content in the three banana orchard farms is sufficient.